437 research outputs found

    Development of porcine embryos reconstituted with somatic cells and enucleated metaphase I and II oocytes matured in a protein-free medium

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    BACKGROUND: Many cloned animals have been created by transfer of differentiated cells at G0/G1 or M phase of the cell cycle into enucleated M II oocytes having high maturation/meiosis/mitosis-promoting factor activity. Because maturation/meiosis/mitosis-promoting factor activity during oocyte maturation is maximal at both M I and M II, M I oocytes may reprogram differentiated cell nuclei as well. The present study was conducted to examine the developmental ability in vitro of porcine embryos reconstructed by transferring somatic cells (ear fibroblasts) into enucleated M I or M II oocytes. RESULTS: Analysis of the cell cycle stages revealed that 91.2 ± 0.2% of confluent cells were at the G0/G1 phase and 54.1 ± 4.4% of nocodazole-treated cells were at the G2/M phase, respectively. At 6 h after activation, nuclear swelling was observed in 50.0-88.9% and 34.4-39.5% of embryos reconstituted with confluent cells and nocodazole-treated cells regardless of the recipient oocytes, respectively. The incidence of both a swollen nucleus and polar body was low (6.3-10.5%) for all nocodazole-treated donor cell regardless of the recipient oocyte. When embryos reconstituted with confluent cells and M I oocytes were cultured, 2 (1.5%) blastocysts were obtained and this was significantly (P < 0.05) lower than that (7.6%) of embryos produced by transferring confluent cells into M II oocytes. No reconstructed embryos developed to the blastocyst stage when nocodazole-treated cells were used as donors. CONCLUSIONS: Porcine M I oocytes have a potential to develop into blastocysts after nuclear transfer of somatic cells

    Tracking aftershock sequences using empirical matched field processing

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    Extensive aftershock sequences present a significant problem to seismological data centres attempting to produce near real-time comprehensive seismic event bulletins. An elevated number of events to process and poorer performance of automatic phase association algorithms can lead to large delays in processing and a greatly increased human workload. Global monitoring is often performed using seismic array stations at considerable distances from the events involved. Empirical matched field processing (EMFP) is a narrow-frequency band array signal processing technique that recognizes the inter-sensor phase and amplitude relations associated with wavefronts approaching a sensor array from a given direction. We demonstrate that EMFP, using a template obtained from the first P arrival from the main shock alone, can efficiently detect and identify P arrivals on that array from subsequent events in the aftershock zone with exceptionally few false alarms (signals from other sources). The empirical wavefield template encodes all the narrow-band phase and amplitude relations observed for the main shock signal. These relations are also often robust and repeatable characteristics of signals from nearby events. The EMFP detection statistic compares the phase and amplitude relations at a given time in the incoming data stream with those for the template and is sensitive to very short-duration signals with the required characteristics. Significant deviations from the plane-wavefront model that typically degrade the performance of standard beamforming techniques can enhance signal characterization using EMFP. Waveform correlation techniques typically perform poorly for aftershocks from large earthquakes due to the distances between hypocentres and the wide range of event magnitudes and source mechanisms. EMFP on remote seismic arrays mitigates these difficulties; the narrow-band nature of the procedure makes arrival identification less sensitive to the signals’ temporal form and spectral content. The empirical steering vectors derived for the main shock P arrival can reduce the frequency dependency of the slowness vector estimates. This property helps us to automatically screen out arrivals from outside of the aftershock zone. Standard array processing pipelines could be enhanced by including both plane-wave and empirical matched field steering vectors. This would maintain present capability for the plane-wave steering vectors and provide increased sensitivity and resolution for those sources for which we have empirical calibrations.Tracking aftershock sequences using empirical matched field processingacceptedVersio

    Altered spring phenology of North American freshwater turtles and the importance of representative populations

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    Globally, populations of diverse taxa have altered phenology in response to climate change. However, most research has focused on a single population of a given taxon, which may be unrepresentative for comparative analyses, and few long-term studies of phenology in ectothermic amniotes have been published. We test for climate- altered phenology using long-term studies (10–36 years) of nesting behavior in 14 populations representing six genera of freshwater turtles (Chelydra, Chrysemys, Kinosternon, Malaclemys, Sternotherus, and Trachemys). Nesting season initiation oc- curs earlier in more recent years, with 11 of the populations advancing phenology. The onset of nesting for nearly all populations correlated well with temperatures during the month preceding nesting. Still, certain populations of some species have not advanced phenology as might be expected from global patterns of climate change. This collection of findings suggests a proximate link between local climate and reproduction that is potentially caused by variation in spring emergence from hibernation, ability to process food, and thermoregulatory opportunities prior to nesting. However, even though all species had populations with at least some evi- dence of phenological advancement, geographic variation in phenology within and among turtle species underscores the critical importance of representative data for accurate comprehensive assessments of the biotic impacts of climate change

    Altered spring phenology of North American freshwater turtles and the importance of representative populations

    Get PDF
    Globally, populations of diverse taxa have altered phenology in response to climate change. However, most research has focused on a single population of a given taxon, which may be unrepresentative for comparative analyses, and few long‐term studies of phenology in ectothermic amniotes have been published. We test for climate‐altered phenology using long‐term studies (10–36 years) of nesting behavior in 14 populations representing six genera of freshwater turtles (Chelydra, Chrysemys, Kinosternon,Malaclemys, Sternotherus, and Trachemys). Nesting season initiation occurs earlier in more recent years, with 11 of the populations advancing phenology. The onset of nesting for nearly all populations correlated well with temperatures during the month preceding nesting. Still, certain populations of some species have not advanced phenology as might be expected from global patterns of climate change. This collection of findings suggests a proximate link between local climate and reproduction that is potentially caused by variation in spring emergence from hibernation, ability to process food, and thermoregulatory opportunities prior to nesting. However, even though all species had populations with at least some evidence of phenological advancement, geographic variation in phenology within and among turtle species underscores the critical importance of representative data for accurate comprehensive assessments of the biotic impacts of climate change

    Geometrical Tachyon Kinks and NS5 Branes

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    We further investigate the NSNS5 ring background using the tachyon map. Mapping the radion fields to the rolling tachyon helps to explain the motion of a probe DpDp-brane in this background. It turns out that the radion field becomes tachyonic when the brane is confined to one dimensional motion inside the ring. We find explicit solutions for the geometrical tachyon field that describe stable kink solutions which are similar to those of the open string tachyon. Interestingly in the case of the geometric tachyon, the dynamics is controlled by a cosine potential. In addition, we couple a constant electric field to the probe-brane, but find that the only stable kink solutions occur when there is zero electric field or a critical field value. We also investigate the behaviour of Non-BPS branes in this background, and find that the end state of any probe brane is that of tachyonic matter 'trapped' around the interior of the ring. We conclude by considering compactification of the ring solution in one of the transverse directions.Comment: Latex, 24 pages, 1 eps fig; clarifying comments added to Section 2; typos correcte

    The Impossibility of a Perfectly Competitive Labor Market

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    Using the institutional theory of transaction cost, I demonstrate that the assumptions of the competitive labor market model are internally contradictory and lead to the conclusion that on purely theoretical grounds a perfectly competitive labor market is a logical impossibility. By extension, the familiar diagram of wage determination by supply and demand is also a logical impossibility and the neoclassical labor demand curve is not a well-defined construct. The reason is that the perfectly competitive market model presumes zero transaction cost and with zero transaction cost all labor is hired as independent contractors, implying multi-person firms, the employment relationship, and labor market disappear. With positive transaction cost, on the other hand, employment contracts are incomplete and the labor supply curve to the firm is upward sloping, again causing the labor demand curve to be ill-defined. As a result, theory suggests that wage rates are always and everywhere an amalgam of an administered and bargained price. Working Paper 06-0

    Fuzzy Sphere Dynamics and Non-Abelian DBI in Curved Backgrounds

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    We consider the non-Abelian action for the dynamics of NDpâ€ČN Dp'-branes in the background of MDpM Dp-branes, which parameterises a fuzzy sphere using the SU(2) algebra. We find that the curved background leads to collapsing solutions for the fuzzy sphere except when we have D0D0 branes in the D6D6 background, which is a realisation of the gravitational Myers effect. Furthermore we find the equations of motion in the Abelian and non-Abelian theories are identical in the large NN limit. By picking a specific ansatz we find that we can incorporate angular momentum into the action, although this imposes restriction upon the dimensionality of the background solutions. We also consider the case of non-Abelian non-BPS branes, and examine the resultant dynamics using world-volume symmetry transformations. We find that the fuzzy sphere always collapses but the solutions are sensitive to the combination of the two conserved charges and we can find expanding solutions with turning points. We go on to consider the coincident NSNS5-brane background, and again construct the non-Abelian theory for both BPS and non-BPS branes. In the latter case we must use symmetry arguments to find additional conserved charges on the world-volumes to solve the equations of motion. We find that in the Non-BPS case there is a turning solution for specific regions of the tachyon and radion fields. Finally we investigate the more general dynamics of fuzzy S2k\mathbb{S}^{2k} in the DpDp-brane background, and find collapsing solutions in all cases.Comment: 49 pages, 3 figures, Latex; Version to appear in JHE
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